大鼠肝脏核糖体中聚（U）聚合酶内源性和外源性RNA引物的比较。
Comparison of endogenous and exogenous RNA primers of poly(U) polymerase in rat hepatic ribosomes.
作者信息
Hayashi T T, MacFarlane K
出版信息
Biochem J. 1979 Mar 1;177(3):895-902. doi: 10.1042/bj1770895.
Abstract
The present work indicates that RNA primer requirements for poly(U) polymerase in the free ribosomes of the rat liver depend upon the degree of enzyme purification. The poly(U) polymerase activity obtained from a crude free ribosomal preparation was compared with the enzymic activity of a partially purified enzyme. After preliminary purification, the enzyme was fractionated by chromatography on Sephadex G-150 and CM-cellulose. Our results demonstrate the presence of several forms of poly(U) polymerase activities, some requiring exogenous RNA and others possessing their own endogenous primer RNA.
摘要
目前的研究表明，大鼠肝脏游离核糖体中聚（U）聚合酶对RNA引物的需求取决于酶的纯化程度。将从粗制游离核糖体制剂中获得的聚（U）聚合酶活性与部分纯化酶的酶活性进行了比较。初步纯化后，通过在Sephadex G - 150和CM - 纤维素上进行色谱分离对该酶进行分级分离。我们的结果证明存在几种形式的聚（U）聚合酶活性，一些需要外源RNA，而另一些则具有自身的内源性引物RNA。
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