Procyk R, Devlin T M, Ch'ih J J
Biochem J. 1979 Feb 15;178(2):501-4. doi: 10.1042/bj1780501.
Fibrinogen synthesis in the intact rat was perturbed by treatment with cycloheximide. Specific radioactivities of fibrinogen in plasma and liver both decreased at 2 h after treatment and increased over 2-fold by 18 h. Labelled-antibody--polyribosome binding experiments showed that more polyribosomes were engaged in fibrinogen synthesis at 18 h after treatment. Radioactivity of plasma fibrinogen chains from untreated control rats showed a constant ratio of A alpha--B beta/gamma = 1.03. At 2 h after cycloheximide treatment the A alpha- and B beta-chains showed the greatest decrease in labelling (A alpha--B beta/gamma = 0.66) and at 18 h all chains were much more labelled (the A alpha--B beta/gamma ratio chainged to 1.39). The observed imbalance in fibrinogen-chain synthesis suggests that cycloheximide has a selective effect on gene expression.
用环己酰亚胺处理完整大鼠会干扰纤维蛋白原的合成。处理后2小时，血浆和肝脏中纤维蛋白原的比放射性均降低，到18小时增加了两倍多。标记抗体-多核糖体结合实验表明，处理后18小时有更多的多核糖体参与纤维蛋白原的合成。未处理对照大鼠血浆纤维蛋白原链的放射性显示Aα-Bβ/γ的恒定比值为1.03。环己酰亚胺处理后2小时，Aα链和Bβ链的标记减少最多（Aα-Bβ/γ = 0.66），18小时时所有链的标记都多得多（Aα-Bβ/γ比值变为1.39）。观察到的纤维蛋白原链合成失衡表明环己酰亚胺对基因表达有选择性作用。
