哺乳动物体内蛋白质合成的调控。环己酰亚胺处理后体内肝脏中蛋白质合成的刺激情况。
Regulation of mammalian protein synthesis in vivo. Stimulated protein synthesis in liver in vivo after cycloheximide treatment.
作者信息
Ch'ih J J, Procyk R, Devlin T M
出版信息
Biochem J. 1977 Mar 15;162(3):501-7. doi: 10.1042/bj1620501.
Abstract
Protein synthesis in rat liver in vivo was measured between 0 and 72 h after administration of a non-lethal dose of cycloheximide. There was a period of inhibition of [3H]leucine incorporation into both intra- and extra-cellular proteins at 2 h after administration of the drug, which was followed by a recovery phase in which amino acid incorporation varied significantly among the various proteins evaluated. At 12 h there was a marked stimulation of incorporation into nascent polypeptides released from polyribosomes and plasma fibrinogen, but incorporation into ribosomal proteins as well as albumin was still inhibited. Between 12 and 48 h, nascent-polypeptide synthesis remained elevated, but ribosomal-protein synthesis recovered slowly from the inhibition to normal rates only, and plasma-albumin synthesis increased slowly to above control values up to 48 h before returning to normal. A differential pattern of incorporation was also observed for incorporation into free and membrane-bound polyribosomes.
摘要
在给予大鼠非致死剂量的环己酰亚胺后0至72小时内，测定其肝脏在体内的蛋白质合成情况。给药后2小时，[3H]亮氨酸掺入细胞内和细胞外蛋白质均受到抑制，随后进入恢复期，在此期间，在所评估的各种蛋白质中，氨基酸掺入情况差异显著。12小时时，从多核糖体释放的新生多肽和血浆纤维蛋白原的掺入显著增加，但核糖体蛋白以及白蛋白的掺入仍受到抑制。在12至48小时之间，新生多肽合成仍处于升高状态，但核糖体蛋白合成仅从抑制状态缓慢恢复至正常速率，血浆白蛋白合成在48小时前缓慢增加至高于对照值，然后才恢复正常。对于游离和膜结合多核糖体的掺入，也观察到了不同的模式。
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