Bertaccini G, Erspamer V, Melchiorri P, Sopranzi N
Br J Pharmacol. 1974 Oct;52(2):219-25. doi: 10.1111/j.1476-5381.1974.tb09703.x.
1 The intravenous infusion of bombesin produced in intact dogs and, more strikingly in dogs provided with gastric fistulae a sharp increase in plasma levels of immunoreactive gastrin and at the same time a stimulation of gastric acid secretion. Gastrin response was correlated with the dose of bombesin from approximately 0.1 mug kg(-1) h(-1) (threshold) to 1 mug kg(-1) h(-1) (maximum gastrin release).2 Atropine and metiamide reduced or inhibited gastric acid secretion stimulated by bombesin, but did not affect the rise in gastrin levels.3 Acidification of the whole stomach or of a perfused antral pouch caused a reduced or delayed response to bombesin. However, the inhibitory effect of acidification could be surmounted by prolonging the duration of bombesin infusion.4 Antrectomy greatly reduced the rise in gastrin levels and the increase in acid gastric secretion produced by bombesin, but left unaffected the gastric secretagogue effect of pentagastrin.5 It is concluded that bombesin is a potent releaser of gastrin from the antral mucosa.6 The possible influence of the renal effects evoked by bombesin in the dog on the gastrin response to the polypeptide is discussed.
向完整的狗静脉输注蛙皮素，更显著的是向有胃瘘的狗静脉输注蛙皮素时，血浆中免疫反应性胃泌素水平急剧升高，同时胃酸分泌受到刺激。胃泌素反应与蛙皮素剂量相关，剂量范围约为0.1微克/千克（-1）小时（阈值）至1微克/千克（-1）小时（最大胃泌素释放）。
阿托品和甲硫咪胺减少或抑制了蛙皮素刺激的胃酸分泌，但不影响胃泌素水平的升高。
全胃或灌注的胃窦袋酸化会导致对蛙皮素的反应降低或延迟。然而，通过延长蛙皮素输注时间可以克服酸化的抑制作用。
胃窦切除术大大降低了蛙皮素引起的胃泌素水平升高和胃酸分泌增加，但不影响五肽胃泌素的促胃液分泌作用。
得出的结论是，蛙皮素是胃窦粘膜释放胃泌素的有效物质。
讨论了蛙皮素在狗身上引起的肾脏效应对该多肽胃泌素反应的可能影响。
