甘油酯甘油合成途径。
Pathways of glyceride glycerol synthesis.
作者信息
Rognstad R, Clark D G, Katz J
出版信息
Biochem J. 1974 May;140(2):249-51. doi: 10.1042/bj1400249.
Abstract
Isolated rat liver parenchymal cells were incubated for various periods with [U-(14)C,2-(3)H]glycerol and the radioisotopic yields in the major products were determined, as well as the (3)H/(14)C ratios in glyceride glycerol and intracellular glycerol phosphate. Under the conditions used (0.1mm-glycerol+10mm-l-lactate or 10mm-glycerol as substrates), only small differences were found between these (3)H/(14)C ratios. The results suggest a minor role for a pathway of glyceride glycerol synthesis involving reduction of acylated dihydroxyacetone phosphate, under these experimental conditions.
摘要
将分离的大鼠肝脏实质细胞与[U-(14)C,2-(3)H]甘油孵育不同时间，测定主要产物中的放射性同位素产率，以及甘油酯甘油和细胞内甘油磷酸中的(3)H/(14)C 比值。在所使用的条件下（以0.1mmol/L甘油+10mmol/L L-乳酸或10mmol/L甘油作为底物），这些(3)H/(14)C 比值之间仅发现微小差异。结果表明，在这些实验条件下，涉及酰化磷酸二羟丙酮还原的甘油酯甘油合成途径作用较小。
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本文引用的文献
1
Acyl dihydroxyacetone phosphate. A rapidly labeled lipid in guinea pig liver mitochondria.
J Biol Chem. 1967 Mar 10;242(5):1074-5.
2
The metabolism of glucose-2-T by adipose tissue.
J Biol Chem. 1969 Jan 10;244(1):99-106.
3
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
4
Tritium isotope effects in the measurement of the glycerol phosphate and dihydroxyacetone phosphate pathways of glycerolipid biosynthesis in rat liver.
Biochem J. 1972 Dec;130(4):1003-12. doi: 10.1042/bj1301003.
5
The metabolism of tritiated glucose by rat adipose tissue.
J Biol Chem. 1966 Aug 10;241(15):3600-10.
6
The pentose cycle, triose phosphate isomerization, and lipogenesis in rat adipose tissue.
J Biol Chem. 1966 Feb 10;241(3):727-40.
7
Enzymic degradation of isotopically labeded compounds. II. Glucose labeled with 14C and tritium.
Anal Biochem. 1970 Mar;34:170-9. doi: 10.1016/0003-2697(70)90098-9.
8
Enzymic degradation of isotopically labeled compounds. I. Degradation of 14C-labeled glycerol.
Anal Biochem. 1970 Mar;34:161-9. doi: 10.1016/0003-2697(70)90097-7.
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