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本文引用的文献

1
Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells.在海拉细胞中证实存在不稳定RNA和核糖体RNA前体。
Proc Natl Acad Sci U S A. 1963 Feb 15;49(2):240-8. doi: 10.1073/pnas.49.2.240.
2
Methylation of 45 s ribosomal RNA precursor in HeLa cells.HeLa细胞中45s核糖体RNA前体的甲基化
J Mol Biol. 1967 Jan 28;23(2):149-61. doi: 10.1016/s0022-2836(67)80023-8.
3
Incorporation of [3-H]uridine and [M3-14-C]methionine into Chinese-hamster cell ribonucleic acid.[3-H]尿苷和[M3-14-C]甲硫氨酸掺入中国仓鼠细胞核糖核酸的研究
Biochim Biophys Acta. 1966 Jun 22;119(3):492-500.
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Methylation of ribosomal RNA precursor and tRNA in rat liver.大鼠肝脏中核糖体RNA前体和转运RNA的甲基化作用
Biochim Biophys Acta. 1968 May 21;157(3):476-92. doi: 10.1016/0005-2787(68)90147-0.
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Denaturation of RNA with dimethyl sulfoxide.用二甲基亚砜使RNA变性。
Biopolymers. 1968 Jun;6(6):793-807. doi: 10.1002/bip.1968.360060604.
6
Variation with temperature of the apparent molecular weight of rat-liver mitochondrial RNA, determined by gel electrophoresis.
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1321-7. doi: 10.1016/0006-291x(70)90233-0.
7
Characterization of RNA molecules restricted to the nucleus in mouse L-cells.小鼠L细胞中定位于细胞核的RNA分子的特性分析。
J Cell Physiol. 1970 Feb;75(1):97-106. doi: 10.1002/jcp.1040750112.
8
Intranuclear accumulation of RNA resembling the smaller ribosomal RNA component.类似于较小核糖体RNA组分的RNA在细胞核内积累。
J Cell Sci. 1970 Jan;6(1):53-72. doi: 10.1242/jcs.6.1.53.
9
Regulation of protein synthesis in HeLa cells: translation at elevated temperatures.HeLa细胞中蛋白质合成的调控：高温下的翻译
J Mol Biol. 1969 Jan;39(2):315-33. doi: 10.1016/0022-2836(69)90320-9.
10
Control of translation by the conformation of messenger RNA.通过信使核糖核酸的构象控制翻译
Proc Natl Acad Sci U S A. 1971 Mar;68(3):570-3. doi: 10.1073/pnas.68.3.570.

异质性核核糖核酸在部分变性和完全变性条件下的行为

The behaviour of heterogeneous nuclear ribonucleic acid in partially and completely denaturing conditions.

作者信息

Bramwell M E

出版信息

Biochem J. 1974 Aug;141(2):477-84. doi: 10.1042/bj1410477.

DOI:10.1042/bj1410477
PMID:4455217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168102/
Abstract

It is shown that the heterogeneous nuclear RNA (HnRNA) synthesized in the presence of actinomycin and at low and high temperatures sediments in low-ionic-strength sucrose gradients between the rRNA components, similar to the unmethylated RNA synthesized under ;step-down' conditions. If the ionic strength is increased then the HnRNA sediments more rapidly than 28S rRNA, with a large proportion about the 45S precursor rRNA position. Initially this was thought to be due to aggregation of the HnRNA; however, centrifugation and electrophoresis in completely denaturing conditions suggest that the molecular weight of this species of RNA is very large The experiments reveal that HnRNA is conformationally unstable relative to the nucleolar RNA and that the slower sedimentation rate of HnRNA in 5mm-EDTA-Tris base-sucrose gradients reflects the greater expansion of the HnRNA relative to the nucleolar RNA. The implications of this finding are discussed.

摘要

结果表明，在放线菌素存在的情况下，于低温和高温条件下合成的不均一核RNA（HnRNA），在低离子强度蔗糖梯度中沉降于rRNA组分之间，类似于在“逐步降低”条件下合成的未甲基化RNA。如果增加离子强度，那么HnRNA的沉降速度比28S rRNA更快，大部分位于约45S前体rRNA的位置。最初认为这是由于HnRNA的聚集；然而，在完全变性条件下的离心和电泳表明，这种RNA的分子量非常大。实验表明，相对于核仁RNA，HnRNA在构象上不稳定，并且HnRNA在5mM - EDTA - Tris碱 - 蔗糖梯度中较慢的沉降速度反映了其相对于核仁RNA更大的伸展。讨论了这一发现的意义。