大鼠肝脏S-腺苷-L-甲硫氨酸脱羧酶的纯化
Purification of rat liver S-adenosyl-L-methionine decarboxylase.
作者信息
Pegg A E
出版信息
Biochem J. 1974 Aug;141(2):581-3. doi: 10.1042/bj1410581.
Abstract
The amount of S-adenosyl-l-methionine decarboxylase present in rat liver was enhanced 17-fold by administration of methylglyoxal bis(guanylhydrazone),* a specific inhibitor of the enzyme. The enzyme was purified 1400-fold in 50% yield from such liver extracts by chromatography on columns of the inhibitor bound to Sepharose. The purified enzyme had no spermidine synthetase activity.
摘要
通过给予甲基乙二醛双（脒腙）（该酶的一种特异性抑制剂），大鼠肝脏中S-腺苷-L-甲硫氨酸脱羧酶的含量增加了17倍。通过在与琼脂糖结合的抑制剂柱上进行层析，从这种肝脏提取物中以50%的产率将该酶纯化了1400倍。纯化后的酶没有亚精胺合成酶活性。
相似文献
1
Purification of rat liver S-adenosyl-L-methionine decarboxylase.大鼠肝脏S-腺苷-L-甲硫氨酸脱羧酶的纯化
Biochem J. 1974 Aug;141(2):581-3. doi: 10.1042/bj1410581.
2
Inhibition of spermidine formation in rat liver and kidney by methylglyoxal bis(guanylhydrazone).甲基乙二醛双（脒腙）对大鼠肝脏和肾脏中精胺形成的抑制作用。
Biochem J. 1973 Mar;132(3):537-40. doi: 10.1042/bj1320537.
3
Stimulation of ornithine decarboxylase activity and inhibition of S-adenosyl-L-methionine decarboxylase activity in leukaemic mice by methylglyoxal bis(guanylhydrazone).甲基乙二醛双（脒腙）对白血病小鼠鸟氨酸脱羧酶活性的刺激作用及S-腺苷-L-甲硫氨酸脱羧酶活性的抑制作用
Biochem J. 1973 Dec;136(4):1121-4. doi: 10.1042/bj1361121.
4
Effect of methylglyoxal bis(guanylhydrazone) on polyamine metabolism in normal and regenerating rat liver and rat thymus.甲基乙二醛双（脒腙）对正常及再生大鼠肝脏和大鼠胸腺中多胺代谢的影响
Biochem J. 1973 Nov;136(3):669-76. doi: 10.1042/bj1360669.
5
Specific inhibition of the enzymic decarboxylation of S-adenosylmethionine by methylglyoxal bis(guanylhydrazone) and related substances.甲基乙二醛双（脒腙）及相关物质对S-腺苷甲硫氨酸酶促脱羧作用的特异性抑制
Biochem J. 1974 May;139(2):351-7. doi: 10.1042/bj1390351.
6
S-adenosylmethionine decarboxylase from baker's yeast.来自面包酵母的S-腺苷甲硫氨酸脱羧酶。
Biochem J. 1975 Oct;151(1):67-73. doi: 10.1042/bj1510067.
7
Purification and characterization of S-adenosyl-L-methionine decarboxylase from rat liver.大鼠肝脏S-腺苷-L-甲硫氨酸脱羧酶的纯化与特性分析
Biochemistry. 1972 Feb 29;11(5):671-7. doi: 10.1021/bi00755a002.
8
The purification of S-adenoxyl-L-methionine decarboxylase from rat liver: inability to separate decarboxylation from spermidine synthesis.从大鼠肝脏中纯化S-腺苷-L-蛋氨酸脱羧酶：无法将脱羧作用与亚精胺合成分离。
Biochem Biophys Res Commun. 1971 Aug 6;44(3):675-81. doi: 10.1016/s0006-291x(71)80136-5.
9
Indirect evidence for a strict negative control of S-adenosyl-L-methionine decarboxylase by spermidine in rat hepatoma cells.在大鼠肝癌细胞中，亚精胺对S-腺苷-L-甲硫氨酸脱羧酶进行严格负调控的间接证据。
Biochem J. 1981 May 15;196(2):411-22. doi: 10.1042/bj1960411.
10
Putrescine-insensitive S-adenosyl-L-methionine decarboxylase from Tetrahymena pyriformis.来自梨形四膜虫的腐胺不敏感型S-腺苷-L-甲硫氨酸脱羧酶。
Acta Chem Scand B. 1975;29(9):932-6. doi: 10.3891/acta.chem.scand.29b-0932.
引用本文的文献
1
S-adenosylmethionine decarboxylase and spermidine synthase from chinese cabbage.大白菜中的S-腺苷甲硫氨酸脱羧酶和亚精胺合酶。
Plant Physiol. 1985 Aug;78(4):784-90. doi: 10.1104/pp.78.4.784.
2
S-adenosylmethionine decarboxylase of corn seedlings.玉米幼苗的 S-腺苷甲硫氨酸脱羧酶。
Plant Physiol. 1980 Dec;66(6):1091-4. doi: 10.1104/pp.66.6.1091.
3
S-Adenosylmethionine decarboxylase from the archaeon Methanococcus jannaschii: identification of a novel family of pyruvoyl enzymes.来自嗜压甲烷球菌的S-腺苷甲硫氨酸脱羧酶：一种新型丙酮酸基酶家族的鉴定。
J Bacteriol. 2000 Dec;182(23):6667-72. doi: 10.1128/JB.182.23.6667-6672.2000.
4
Differences between tissues in response of S-adenosylmethionine decarboxylase to administration of polyamines.组织间S-腺苷甲硫氨酸脱羧酶对多胺给药反应的差异。
Biochem J. 1981 Dec 15;200(3):629-37. doi: 10.1042/bj2000629.
5
Polyamine synthesis in mammalian tissues. Isolation and characterization of spermidine synthase from bovine brain.哺乳动物组织中的多胺合成。牛脑亚精胺合酶的分离与鉴定。
Biochem J. 1984 May 1;219(3):991-1000. doi: 10.1042/bj2190991.
6
Comparison of inhibitors of S-adenosylmethionine decarboxylase from different species.不同物种间S-腺苷甲硫氨酸脱羧酶抑制剂的比较。
Biochem J. 1983 Aug 1;213(2):495-502. doi: 10.1042/bj2130495.
7
S-adenosylmethionine decarboxylase from baker's yeast.来自面包酵母的S-腺苷甲硫氨酸脱羧酶。
Biochem J. 1975 Oct;151(1):67-73. doi: 10.1042/bj1510067.
8
Role of pyridoxal phosphate in mammalian polyamine biosynthesis. Lack of requirement for mammalian S-adenosylmethionine decarboxylase activity.磷酸吡哆醛在哺乳动物多胺生物合成中的作用。对哺乳动物S-腺苷甲硫氨酸脱羧酶活性的需求缺失。
Biochem J. 1977 Jul 15;166(1):81-8. doi: 10.1042/bj1660081.
9
The decarboxylation of S-adenosylmethionine by detergent-treated extracts of rat liver.用经去污剂处理的大鼠肝脏提取物对S-腺苷甲硫氨酸进行脱羧反应。
Biochem J. 1979 Jun 15;180(3):515-22. doi: 10.1042/bj1800515.
10
Isolation and characterization of Saccharomyces cerevisiae mutants deficient in S-adenosylmethionine decarboxylase, spermidine, and spermine.缺乏S-腺苷甲硫氨酸脱羧酶、亚精胺和精胺的酿酒酵母突变体的分离与鉴定
J Bacteriol. 1978 Apr;134(1):208-13. doi: 10.1128/jb.134.1.208-213.1978.
本文引用的文献
1
Polyamine synthesis and accumulation in the castrated rat uterus after estradiol-17-beta stimulation.雌二醇-17-β刺激后去势大鼠子宫中的多胺合成与积累
Endocrinology. 1971 Jun;88(6):1397-403. doi: 10.1210/endo-88-6-1397.
2
On the purification of L-ornithine decarboxylase from rat prostate and effects of thiol compounds on the enzyme.大鼠前列腺L-鸟氨酸脱羧酶的纯化及硫醇化合物对该酶的影响
J Biol Chem. 1971 Mar 25;246(6):1725-32.
3
4
Purification and characterization of S-adenosyl-L-methionine decarboxylase from rat liver.大鼠肝脏S-腺苷-L-甲硫氨酸脱羧酶的纯化与特性分析
Biochemistry. 1972 Feb 29;11(5):671-7. doi: 10.1021/bi00755a002.
5
Separation and partial purification of S-adenosylmethionine decarboxylase and spermidine and spermine synthases from rat liver.从大鼠肝脏中分离并部分纯化S-腺苷甲硫氨酸脱羧酶、亚精胺和精胺合酶。
Biochem Biophys Res Commun. 1972 Jan 31;46(2):341-8. doi: 10.1016/s0006-291x(72)80144-x.
6
Polyamine synthesis in the regenerating rat liver: stimulation of S-adenosyl methionine decarboxylase, and spermidine and spermine synthases after partial hepatectomy.再生大鼠肝脏中的多胺合成：部分肝切除术后S-腺苷甲硫氨酸脱羧酶、亚精胺和精胺合成酶的刺激作用
Biochim Biophys Acta. 1972 Jun 26;273(1):84-90. doi: 10.1016/0304-4165(72)90194-8.
7
Purification of adenosylmethionine decarboxylase from Escherichia coli W: evidence for covalently bound pyruvate.从大肠杆菌W中纯化腺苷甲硫氨酸脱羧酶：共价结合丙酮酸的证据。
J Biol Chem. 1970 Apr 25;245(8):2132-9.
8
On the role of S-adenosyl-L-methionine in the biosynthesis of spermidine by rat prostate.S-腺苷-L-甲硫氨酸在大鼠前列腺生物合成亚精胺中的作用
J Biol Chem. 1969 Feb 25;244(4):682-93.
9
S-adenosyl-L-methionine decarboxylase during lymphocyte transformation: decreased degradation in the presence of a specific inhibitor.淋巴细胞转化过程中的S-腺苷-L-蛋氨酸脱羧酶：在存在特异性抑制剂的情况下降解减少。
Biochem Biophys Res Commun. 1973 Jun 8;52(3):1020-5. doi: 10.1016/0006-291x(73)91039-5.
10
Paradoxical enhancement of S-adenosylmethionine decarboxylase in rat tissues following administration of the specific inhibitor methyl glyoxal bis(guanylhydrazone).在给予特异性抑制剂甲基乙二醛双（脒腙）后大鼠组织中S-腺苷甲硫氨酸脱羧酶的反常增强。
Biochem Biophys Res Commun. 1973 May 15;52(2):696-701. doi: 10.1016/0006-291x(73)90768-7.
Zotero 插件