Grosse G, Lindner G
Z Mikrosk Anat Forsch. 1974;88(4):705-12.
Cell cultures from hippocampus of 16 and 17 days old embryonal rats were cultivated up to 4 weeks. After 24 hours in vitro on 18.4 percent of cells and after 5 days in vitro on 70 percent of cells processes could be recognized. These are neuroblasts. The cells reaggregated. Nerve fibers after 4 weeks in vitro are 200 to 300 mum long. Small and big neurons with 12 mum to 26 mum diameters of perikarya, bi- and multipolar neurons after 4 weeks in vitro were observed. In cultures and meningothel-monolayer developed. Maintenance and differentiation of cultures are possible only by sowing in at least 60,000 cells/ml medium. The advantage of cell culture opposite to organ culture exists in experiments with immediate selective influence.
取自16至17日龄胚胎大鼠海马的细胞培养物培养了4周。体外培养24小时后，18.4%的细胞出现突起；体外培养5天后，70%的细胞出现突起。这些是神经母细胞。细胞重新聚集。体外培养4周后，神经纤维长度为200至300微米。观察到体外培养4周后有直径为12至26微米的大小神经元，双极和多极神经元。在培养物中形成了脑膜上皮单层。只有以至少60,000个细胞/毫升培养基的接种量播种，培养物的维持和分化才有可能。与器官培养相比，细胞培养的优势在于可进行具有直接选择性影响的实验。
