Grosse G, Lindner G
Z Mikrosk Anat Forsch. 1982;96(3):527-36.
150 hippocampal explants from fetal and neonatal rats were cultivated with semisynthetic media in maximow chambers without movement and in roller tubes by rotation in the virutor up to 3 days. The cultures were fixed in Bouin, cuted 15 microns and coloured with the method of Klüver-Barrera or haemalaun. Histological examination of the explants revealed that hippocampi cultured in maximow chambers without movement possess an organisation similar to that observed in situ. Experiments with rotated explants of hippocampus indicate that the structure of hippocampus in vitro is changed. Especially neurons are degenerated and the migration of cells is altered. Finally the value of these two methodological cultivations for neurobiology is discussed.
将150个来自胎鼠和新生鼠的海马体组织块，在无移动的马克西莫培养箱中，使用半合成培养基进行培养，并在旋转器中通过旋转培养3天。培养物用布因氏液固定，切成15微米厚，并用克吕弗-巴雷拉法或苏木精染色。对组织块的组织学检查显示，在无移动的马克西莫培养箱中培养的海马体具有与原位观察到的相似的组织结构。对旋转培养的海马体组织块进行的实验表明，体外培养的海马体结构发生了变化。特别是神经元发生了退化，细胞迁移也发生了改变。最后讨论了这两种培养方法在神经生物学中的价值。
