高度多样化载体两性电解质的合成。在Pi系统（α-1-抗胰蛋白酶基因多态性）中对等电聚焦分辨率的评估。

Synthesis of highly diversified carrier ampholytes. Evaluation of the resolving power of isoelectric focusing in the Pi system (alpha-1-antitrypsin genetic polymorphism).

作者信息

Charlionet R, Martin J P, Sesboüé R, Madec P J, Lefebvre F

出版信息

J Chromatogr. 1979 Aug 1;176(1):89-101. doi: 10.1016/s0021-9673(00)92089-2.

DOI:10.1016/s0021-9673(00)92089-2

Abstract

The use of condensing reagents such as epoxypropanol, diepoxyoctane, acrylamide and N,N'-methylenebisacrylamide in the synthesis of carrier ampholytes increased the diversity of amphoteric components. The quality of these synthetic carrier ampholytes has been tested in the separation of variants of alpha-1-anti-trypsin, a genetic polymorphism called the Pi system. A resolving power of the order of 0.005 pH unit was obtained.

摘要

在载体两性电解质的合成中使用诸如环氧丙醇、二环氧辛烷、丙烯酰胺和N,N'-亚甲基双丙烯酰胺等缩合试剂增加了两性组分的多样性。这些合成载体两性电解质的质量已在α-1-抗胰蛋白酶变体的分离中进行了测试，α-1-抗胰蛋白酶变体是一种称为Pi系统的遗传多态性。获得了约0.005 pH单位的分辨能力。