Chen L H, Chang H M
Int J Vitam Nutr Res. 1979;49(1):87-91.
Two groups of weanling male Hartley guinea pigs maintained on vitamin E deficient diet were supplemented with 0.4 I.U./100 g body weight/day of vitamin E and either 2 (Group A) or 10 (Group B) mg/100 g body weight/day of vitamin C for 5 weeks. As compared to Group A, the degree of erythrocyte hemolysis and liver TBAR level of Group B were significantly increased while plasma vitamin E and erythrocyte GSH levels were significantly decreased. In another experiment, two groups of guinea pigs were given 0.8 I.U./100 g body weight/day of vitamin E and 2 (Group C) or 30 mg/100 g body weight/day (Group D) of vitamin C. Levels of plasma vitamin E and erythrocyte GSH of Group D were significantly lower than those of Group C: however, erythrocyte hemolysis and liver TBAR were not affected by the level of vitamin C supplementation. The results suggest that the high levels of vitamin C supplementation lowered tissue antioxidant potential of animal when vitamin E was marginally adequate, and the hemolytic and peroxidizing effect of high level of vitamin C may be counteracted by increasing the level of vitamin E.
两组断奶雄性哈特利豚鼠维持在维生素E缺乏饮食状态下，分别补充0.4国际单位/100克体重/天的维生素E以及2毫克/100克体重/天（A组）或10毫克/100克体重/天（B组）的维生素C，持续5周。与A组相比，B组的红细胞溶血程度和肝脏硫代巴比妥酸反应物（TBAR）水平显著升高，而血浆维生素E和红细胞谷胱甘肽（GSH）水平显著降低。在另一项实验中，两组豚鼠分别给予0.8国际单位/100克体重/天的维生素E以及2毫克/100克体重/天（C组）或30毫克/100克体重/天（D组）的维生素C。D组的血浆维生素E和红细胞GSH水平显著低于C组；然而，红细胞溶血和肝脏TBAR不受维生素C补充水平的影响。结果表明，当维生素E仅处于勉强充足水平时，高剂量补充维生素C会降低动物组织的抗氧化潜能，而增加维生素E水平可能会抵消高剂量维生素C的溶血和过氧化作用。
