David-West T S, Isoun T T, David-West A S
Br J Exp Pathol. 1974 Aug;55(4):421-6.
The effect of the route of inoculation of Dugbe virus (a Nigerian tick-borne virus) in baby mice was investigated. Striking differences were observed in the onset of morbidity, organ virus titres at the terminal stages of the infection, the pathology of the various organs and the haematological picture, depending upon whether the animals were infected intracerebrally (i.c.) or intraperitoneally (i.p.). Mice infected i.c. became moribund 2-3 days earlier than those infected i.p. Furthermore, the virus titres of the various organs were generally higher by 1-2 log in the former group of mice. The most distinguishing feature of the brain pathology of i.p. inoculated mice was the presence of intranuclear inclusion bodies in the neurons of the hippocampus. In contrast, there were no inclusions after i.c. inoculation. There was depletion of mature lymphocytes from germinal centres of the spleen in i.c. inoculated mice, whereas in the i.p. inoculated mice there was lymphoid hyperplasia. At the terminal stages of i.c. infection there was a tremendous increase in neutrophils, while in i.p. inoculated mice there was an increase in plasmacytoid lymphocytes. Anaemia was another haematological feature of i.p. inoculated mice; on the other hand, there was no anaemia in i.c. inoculated mice.
研究了杜贝病毒（一种尼日利亚蜱传病毒）接种途径对幼鼠的影响。根据动物是通过脑内（i.c.）还是腹腔内（i.p.）感染，在发病开始、感染末期器官病毒滴度、各器官病理学以及血液学表现方面观察到了显著差异。脑内感染的小鼠比腹腔内感染的小鼠早2 - 3天濒死。此外，在前一组小鼠中，各器官的病毒滴度通常高1 - 2个对数。腹腔接种小鼠脑病理学最显著的特征是海马神经元中存在核内包涵体。相比之下，脑内接种后没有包涵体。脑内接种的小鼠脾脏生发中心成熟淋巴细胞减少，而腹腔接种的小鼠则出现淋巴样增生。在脑内感染末期，中性粒细胞大量增加，而腹腔接种的小鼠浆细胞样淋巴细胞增加。贫血是腹腔接种小鼠的另一个血液学特征；另一方面，脑内接种的小鼠没有贫血。
