Cook R G, Siegelman M H, Capra J D, Uhr J W, Vitetta E S
J Immunol. 1979 Jun;122(6):2232-7.
Murine Ia alloantigens encoded by the I-A and I-E/C subregions were isolated from radiolabeled splenic lysates and examined by NH2-terminal sequence analysis. Haplotype-associated sequence variation was detected in the beta, but not alpha, subunits of both the A and E/C alloantigens. The A beta-polypeptides from k and b haplotypes show four differences in the 12 positions compared, whereas the E/C beta-polypeptides from k and r haplotypes show two differences in the 13 positions compared. No sequence variation was detected between the Ak and Ab alpha-chains (six positions compared) or between the E/Ck and E/Cr alpha-chains (11 positions compared). Homology relationships between these murine Ia alloantigens and the human Ia (DR) alloantigens are also presented.
从经放射性标记的脾裂解物中分离出由I - A和I - E/C亚区编码的小鼠Ia同种异体抗原，并通过氨基末端序列分析进行检测。在A和E/C同种异体抗原的β亚基而非α亚基中检测到单倍型相关的序列变异。来自k和b单倍型的Aβ多肽在比较的12个位置上有4个差异，而来自k和r单倍型的E/Cβ多肽在比较的13个位置上有2个差异。在Ak和Abα链之间（比较6个位置）或E/Ck和E/Crα链之间（比较11个位置）未检测到序列变异。还展示了这些小鼠Ia同种异体抗原与人类Ia（DR）同种异体抗原之间的同源关系。
