Palmer J P, Werner P L, Hollander P, Ensinck J W
Metabolism. 1979 May;28(5):549-52. doi: 10.1016/0026-0495(79)90196-3.
To evaluate the influence of the parasympathetic nervous system on human glucagon secretion, we have measured the plasma immunoreactive glucagon (IRG) levels after the administration of edrophonium, bethanechol chloride, and 2-deoxyglucose, and have compared the IRG responses to hypoglycemia in normal, atropinized, and vagotomized man. Edrophonium administered i.v. and bethanechol chloride administered s.c. did not affect IRG levels. Two-deoxy-glucose resulted in symptomatic neuroglucopenia with resultant vagal discharge, as evidenced by increased gastric acid secretion; but no changes in IRG concentrations were observed. The IRG response to insulin-induced hypoglycemia in normal subjects was not influenced by the administration of atropine. In seven subjects with a truncal vagotomy and no increased gastric acid secretion during insulin-induced hypoglycemia, the IRG increases were indistinguishable from those of control subjects in terms of timing, peak level obtained, or total glucagon response. We conclude that the cholinergic system is unlikely to play an important role in modulating glucagon secretion in man.
为评估副交感神经系统对人体胰高血糖素分泌的影响，我们测定了静脉注射依酚氯铵、皮下注射氯贝胆碱和2-脱氧葡萄糖后血浆免疫反应性胰高血糖素（IRG）水平，并比较了正常、阿托品化和迷走神经切断的人体对低血糖的IRG反应。静脉注射依酚氯铵和皮下注射氯贝胆碱均未影响IRG水平。2-脱氧葡萄糖导致有症状的神经低血糖症，并伴有迷走神经放电，胃酸分泌增加证明了这一点；但未观察到IRG浓度有变化。正常受试者对胰岛素诱导的低血糖的IRG反应不受阿托品给药的影响。在7名接受了迷走神经干切断术且在胰岛素诱导的低血糖期间胃酸分泌未增加的受试者中，就发生时间、获得的峰值水平或胰高血糖素总反应而言，IRG的增加与对照受试者无法区分。我们得出结论，胆碱能系统不太可能在调节人体胰高血糖素分泌中起重要作用。
