自溶素参与苯唑西林对金黄色葡萄球菌杀菌作用的证据。
Evidence for participation of autolysins in bactericidal action of oxacillin on Staphylococcus aureus.
作者信息
Best G K, Best N H, Koval A V
出版信息
Antimicrob Agents Chemother. 1974 Dec;6(6):825-30. doi: 10.1128/AAC.6.6.825.
Abstract
A comparison of the autolytic enzyme activity in Staphylococcus aureus strains that differ markedly in their rates of lysis and killing after exposure to oxacillin has been made. Log-phase cells of the clinical isolate that is tolerant to oxacillin inhibition were found to contain a level of autolytic enzyme activity comparable to that in a sensitive strain. This autolysin from log-phase cells was recovered after a single freeze-thaw cycle and assayed by using both native and penicillin (un-cross-linked) mureins. These same assays, however, revealed a significant difference in autolysin activity extractable from the two strains if the cells were inhibited by oxacillin. Under these conditions, the S. aureus strain that is susceptible to the killing and lytic effects of oxacillin had considerably more activity on penicillin murein than did the tolerant organism. These results provide evidence that hydrolytic enzymes on the cell surface are required to augment the wall damage initiated by oxacillin and other beta-lactam antibotics to produce a bactericidal effect.
摘要
对暴露于苯唑西林后裂解和杀灭速率存在显著差异的金黄色葡萄球菌菌株中的自溶酶活性进行了比较。发现对苯唑西林抑制具有耐受性的临床分离株的对数期细胞所含的自溶酶活性水平与敏感菌株相当。经过单次冻融循环后，从对数期细胞中回收了这种自溶素，并使用天然和青霉素（未交联）胞壁质进行测定。然而，如果细胞受到苯唑西林抑制，这些相同的测定结果显示，从这两种菌株中可提取的自溶素活性存在显著差异。在这些条件下，对苯唑西林的杀伤和裂解作用敏感的金黄色葡萄球菌菌株对青霉素胞壁质的活性比对耐受性菌株高得多。这些结果提供了证据，表明细胞表面的水解酶是增强由苯唑西林和其他β-内酰胺类抗生素引发的细胞壁损伤以产生杀菌作用所必需的。
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