Borkowski A, Muquardt C
N Engl J Med. 1979 Aug 9;301(6):298-302. doi: 10.1056/NEJM197908093010603.
To determine whether ectopic secretion of a protein hormone can occur normally, we studied plasma from normal, nonpregnant subjects for the presence of a placental hormone, human chorionic gonadotropin. We extracted and purified this hormone from other plasma proteins. We identified the hormone in the final residue on the basis of its dose-response curves in a specific radioimmunoassay and calculated the plasma concentration after correction for losses. Because this assay is sensitive to concentrations as low as 2 pg per milliliter, human chorionic gonadotropin could be detected in the plasma of 12 of 16 blood donors; the median concentration was 19 pg per milliliter (range, less than 2 to 361). This immunologic human chorionic gonadotropin was further characterized from a pool of normal plasma by gel filtration on Sephadex G-100 and was found to be identical to the standard form of the hormone. The concentration in this pool from 13 normal men was 18 pg per milliliter. The source of this ectopic hormone production is unknown, but may be normal, rapidly proliferating nonmalignant cells.
为了确定蛋白质激素的异位分泌是否能正常发生，我们研究了正常非妊娠受试者血浆中胎盘激素人绒毛膜促性腺激素的存在情况。我们从其他血浆蛋白中提取并纯化了这种激素。我们根据其在特定放射免疫分析中的剂量反应曲线，在最终残余物中鉴定出该激素，并校正损失后计算血浆浓度。由于该分析对低至每毫升2皮克的浓度敏感，在16名献血者中的12人血浆中检测到人绒毛膜促性腺激素；中位数浓度为每毫升19皮克（范围为小于2至361）。通过在葡聚糖凝胶G - 100上进行凝胶过滤，从正常血浆池中进一步鉴定了这种免疫性人绒毛膜促性腺激素，发现其与该激素的标准形式相同。来自13名正常男性的该血浆池中的浓度为每毫升18皮克。这种异位激素产生的来源尚不清楚，但可能是正常的、快速增殖的非恶性细胞。
