通过免疫溶血法纯化促红细胞生成素反应性细胞。
Purification of erythropoietin-responsive cells by immune hemolysis.
作者信息
Cantor L N, Morris A J, Marks P A, Rifkind R A
出版信息
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1337-41. doi: 10.1073/pnas.69.6.1337.
Abstract
Immature erythroid cell precursors from 12- and 13-day fetal mouse livers were concentrated by differential antibody-mediated hemolysis. The effect of erythropoietin on these purified erythroid cell precursors was studied in vitro. Addition of erythropoietin stimulates erythroid cells to proliferate, and to differentiate to cells that actively synthesize hemoglobin. This erythropoietin-induced erythropoiesis is sustained in culture for at least 48 hr.
摘要
通过差异抗体介导的溶血法富集来自12日龄和13日龄胎鼠肝脏的未成熟红细胞前体细胞。体外研究了促红细胞生成素对这些纯化的红细胞前体细胞的作用。添加促红细胞生成素可刺激红细胞增殖，并分化为能积极合成血红蛋白的细胞。这种促红细胞生成素诱导的红细胞生成在培养中可持续至少48小时。
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