胸腺素（一种胸腺分泌的激素）的纯化及生物活性
Purification and biological activity of thymosin, a hormone of the thymus gland.
作者信息
Goldstein A L, Guha A, Zatz M M, Hardy M A, White A
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1800-3. doi: 10.1073/pnas.69.7.1800.
Abstract
The purification and chemical properties of thymosin, obtained from bovine thymus tissue, are described. The biological activity of the thymic hormone has been assessed by a newly developed rosette assay, which permits measurement of thymus-dependent lymphoid cells. Thymosin activity is associated with a physico-chemically homogeneous protein of molecular weight 12,600. The hormonal activity is evident in in vitro incubation assay, after injection into adult thymectomized mice, and in prolonging survival of neonatally thymectomized mice and the reconstitution of their response to a skin allograft.
摘要
本文描述了从牛胸腺组织中提取的胸腺素的纯化方法及其化学性质。胸腺激素的生物活性已通过一种新开发的玫瑰花结试验进行评估，该试验可用于测定胸腺依赖性淋巴细胞。胸腺素活性与一种分子量为12,600的物理化学性质均一的蛋白质相关。在体外培养试验中、注射到成年胸腺切除小鼠体内后、以及延长新生胸腺切除小鼠的存活时间并恢复其对同种异体皮肤移植的反应方面，这种激素活性均很明显。
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Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (thymosin).胸腺淋巴细胞生成因子（胸腺素）的制备、测定及部分纯化
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Antigen recognition by T lymphocytes. II. Similar effects of azathioprine, antilymphocyte serum, and anti-theta serum on rosette-forming lymphocytes in normal and neonatally thymectomized mice.T淋巴细胞的抗原识别。II. 硫唑嘌呤、抗淋巴细胞血清和抗θ血清对正常及新生期胸腺切除小鼠中形成玫瑰花结的淋巴细胞的类似作用。
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Proc Natl Acad Sci U S A. 1971 Nov;68(11):2734-8. doi: 10.1073/pnas.68.11.2734.
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