Colten H R
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2233-6. doi: 10.1073/pnas.69.8.2233.
Peritoneal cells isolated from guinea pigs homozygous for a deficiency of the fourth component of complement (C4), produce in vitro a factor that induces the synthesis and secretion of functionally active human C4 by a human cell. This factor appeared to switch on production of C4 in the responsive cell without affecting total protein synthesis. The regulator factor is not species specific, inasmuch as guinea pig regulator affected a corresponding human gene function. Both synthesis of the factor and the response to it were inhibited by actinomycin D. The amount of regulator recovered from genetically deficient cells was about 5-10 times that recovered from normal guinea pig cells.
从补体第四成分（C4）缺乏的纯合豚鼠中分离出的腹膜细胞，在体外产生一种因子，该因子可诱导人细胞合成并分泌功能活性的人C4。该因子似乎在不影响总蛋白质合成的情况下开启了反应性细胞中C4的产生。调节因子不是物种特异性的，因为豚鼠调节因子影响了相应的人类基因功能。放线菌素D可抑制该因子的合成及其反应。从基因缺陷细胞中回收的调节因子量约为从正常豚鼠细胞中回收量的5至10倍。
