核小体与DNA溶液结构和动力学的31P核磁共振研究。
31P NMR studies of the solution structure and dynamics of nucleosomes and DNA.
作者信息
Klevan L, Armitage I M, Crothers D M
出版信息
Nucleic Acids Res. 1979 Apr;6(4):1607-16. doi: 10.1093/nar/6.4.1607.
Abstract
31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31P chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31P spin-lattice relaxation times T1 and 31P-[H] nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 x 10(-10) sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.
摘要
对核小体中140个碱基对的DNA片段以及溶液中游离的该片段进行的31P核磁共振研究表明，当DNA包装成核小体时，核苷酸间31P化学位移或线宽没有可检测到的变化。对31P自旋-晶格弛豫时间T1和31P-[H]核Overhauser增强效应的测量显示，无论是溶液中游离的还是与核小体核心蛋白结合的双螺旋DNA，都存在相关时间约为4×10(-10)秒的内部运动。这一结果意味着双螺旋DNA中的动态流动性比之前认为的更大。
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