Spodheim-Maurizot M, Saint-Ruf G, Leng M
Nucleic Acids Res. 1979 Apr;6(4):1683-94. doi: 10.1093/nar/6.4.1683.
The conformation of DNA modified in vitro by the covalent binding of N-OH-AF was investigated by ultraviolet absorbance, circular dichroism and by radioimmunoassay using specific antibodies against Guo-AAF and nDNA-AAF. The results obtained by both physico-chemical and immunological methods are in agreement with a model involving destabilized regions in the double helical DNA around the carcinogen molecule in which, however, the -AF residues are stacked to the adjacent nucleotides. The RIA results show that the -AF residues are less accessible to antibodies in native than in denatured DNA-AF and thus suggest -AF residues partially buried in the interior of the DNA helix. The present model is compared to the one existing for DNA modified by reaction with N-AcO-AAF (DNA-AAF) (1,2).
通过紫外吸收、圆二色性以及使用针对鸟嘌呤-AAF和天然DNA-AAF的特异性抗体进行放射免疫测定，研究了通过N-OH-AF共价结合在体外修饰的DNA的构象。通过物理化学和免疫学方法获得的结果与一个模型相符，该模型涉及致癌物分子周围双螺旋DNA中不稳定区域，然而，-AF残基与相邻核苷酸堆积在一起。放射免疫测定结果表明，与变性的DNA-AF相比，天然DNA-AF中的-AF残基较难被抗体识别，因此表明-AF残基部分埋在DNA螺旋内部。将当前模型与通过与N-AcO-AAF反应修饰的DNA（DNA-AAF）（1,2）所存在的模型进行了比较。
