多聚（dG-dC）·多聚（dG-dC）的构象：被致癌物N-乙酰氧基-N-乙酰-2-氨基芴和N-羟基-N-2-氨基芴修饰后的情况
Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
作者信息
Sage E, Leng M
出版信息
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4597-601. doi: 10.1073/pnas.77.8.4597.
Abstract
Poly(dG-dC) . poly(dG-dC) was modified by reaction with N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF). Two samples with 6.6% and 8.5% modified bases were prepared. The modified bases are randomly distributed along the polymer chain, as deduced from competition experiments between antibodies against N-2-(guanosin-8-yl)-acetylaminofluorene, modified poly(dG-dC) . poly(dG-dC), and modified DNAs. Circular dichroism studies show that poly(dG-dC) . poly(dG-dC) modified by N-AcO-AAF is much more sensitive to the addition of alcohol than poly(dG-dC) . poly(dG-dC). In about 50% (vol/vol) alcohol, both polynucleotides have the same conformation, which is the Z form or a Z-like form. Moreover, in low salt and in the absence of alcohol, poly(dG-dC) . poly(dG-dC) modified by N-AcO-AAF is partially in the Z form. Poly(dG-dC) . poly(dG-dC) modified by N-hydroxy-N-2-aminofluorene can also adopt the Z form, but the transition is induced at a higher percentage than that of poly(dG-dC) . poly(dG-dC) modified by N-AcO-AAF. In low salt and in the absence of alcohol, no Z form was detected in poly(dG-dC) . poly(dG-dC) modified by N-hydroxy-N-2-aminofluorene.
摘要
聚（dG-dC）·聚（dG-dC）通过与N-乙酰氧基-N-乙酰基-2-氨基芴（N-AcO-AAF）反应进行修饰。制备了两个修饰碱基含量分别为6.6%和8.5%的样品。从针对N-2-（鸟苷-8-基）-乙酰氨基芴、修饰的聚（dG-dC）·聚（dG-dC）和修饰DNA的抗体之间的竞争实验推断，修饰碱基沿聚合物链随机分布。圆二色性研究表明，N-AcO-AAF修饰的聚（dG-dC）·聚（dG-dC）比聚（dG-dC）·聚（dG-dC）对添加醇更为敏感。在约50%（体积/体积）的醇中，两种多核苷酸具有相同的构象，即Z型或类Z型。此外，在低盐和无醇的情况下，N-AcO-AAF修饰的聚（dG-dC）·聚（dG-dC）部分呈Z型。N-羟基-N-2-氨基芴修饰的聚（dG-dC）·聚（dG-dC）也可呈现Z型，但转变所需的百分比高于N-AcO-AAF修饰的聚（dG-dC）·聚（dG-dC）。在低盐和无醇的情况下，未检测到N-羟基-N-2-氨基芴修饰的聚（dG-dC）·聚（dG-dC）呈Z型。
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