Rhodes D
Nucleic Acids Res. 1979;6(5):1805-16. doi: 10.1093/nar/6.5.1805.
In this paper we describe a detailed investigation of the reconstitution of nucleosome cores from poly (dA-dT) and the octamer of histones. We also attempted the reconstitution from the copolymers poly dA.poly dT, poly dG.poly dC and poly (dG-dC). The repeat of the reconstituted chromatin fibre is discussed. The micrococcal nuclease released poly (dA-dT) core particle is found to contain a considerably narrower DNA size distribution that of the native random DNA nucleosome core (12). In addition we have succeeded in obtaining small crystals of the poly (dA-dT) nucleosome core. The DNAase I digestion pattern of the poly (dA-dT) containing nucleosome core is presented. The periodicity of DNAase I cutting sites is found to be about 10.5 bases and is similar to that of the native nucleosome core (12, 13).
在本文中，我们描述了对由聚（dA-dT）和组蛋白八聚体重构核小体核心的详细研究。我们还尝试从共聚物聚dA·聚dT、聚dG·聚dC和聚（dG-dC）进行重构。讨论了重构染色质纤维的重复序列。发现微球菌核酸酶释放的聚（dA-dT）核心颗粒所含DNA的大小分布比天然随机DNA核小体核心（12）的要窄得多。此外，我们成功获得了聚（dA-dT）核小体核心的小晶体。给出了含聚（dA-dT）核小体核心的DNA酶I消化模式。发现DNA酶I切割位点的周期性约为10.5个碱基，与天然核小体核心（12, 13）相似。
