Bürk R R
Proc Natl Acad Sci U S A. 1973 Feb;70(2):369-72. doi: 10.1073/pnas.70.2.369.
When a monolayer culture of normal Balb/c3T3 cells is wounded by scraping away part of the cell sheet, the cells do not migrate into the cleared area unless there is serum in the culture medium. By contrast, SV40-transformed Balb/c3T3 cells do migrate into the wound area without serum. A quantitative assay for the migration of Balb/c3T3 cells into wounds is described. This assay is used in the partial purification of a migration factor released into serum-free medium by SV28 cells. SV28 is a line of BHK21/13 hamster cells transformed by SV40 chosen for its malignancy. The most purified fractions have about 1500 times the specific activity of whole calf serum. These fractions have an activity that promotes overgrowth of Balb/c3T3 cells to high density and an activity that prolongs cell survival without serum. The SV28 migration factor is not extractable from the medium of untransformed BHK21/13 cells or from serum. This migration factor might contribute to the malignancy of SV28 cells.
当通过刮去部分细胞片使正常Balb/c3T3细胞的单层培养物形成伤口时，除非培养基中有血清，否则细胞不会迁移到清除区域。相比之下，SV40转化的Balb/c3T3细胞在无血清的情况下确实会迁移到伤口区域。本文描述了一种用于定量检测Balb/c3T3细胞向伤口迁移的方法。该方法用于部分纯化SV28细胞释放到无血清培养基中的迁移因子。SV28是由SV40转化的BHK21/13仓鼠细胞系，因其恶性程度而被选用。纯化程度最高的组分的比活性约为全小牛血清的1500倍。这些组分具有促进Balb/c3T3细胞过度生长至高密度的活性以及在无血清条件下延长细胞存活的活性。SV28迁移因子不能从未转化的BHK21/13细胞的培养基或血清中提取出来。这种迁移因子可能促成了SV28细胞的恶性程度。
