小鼠3T3成纤维细胞对血清因子的迁移反应。
Migration of mouse 3T3 fibroblasts in response to a serum factor.
作者信息
Lipton A, Klinger I, Paul D, Holley R W
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2799-801. doi: 10.1073/pnas.68.11.2799.
Abstract
A serum factor that promotes migration of (mouse) 3T3 fibroblast cells is shown to be distinct from the growth-promoting and cell-survival factors of serum. The factor promotes migration of cells from the edge of a wound in confluent 3T3 cells, but cell migration under these conditions does not lead to the initiation of DNA synthesis. Subsequent addition of serum initiates DNA synthesis in the migrating cells. The results establish that breaking contacts between quiescent 3T3 cells is not sufficient to initiate DNA synthesis. The DNA synthesis observed in migrating 3T3 cells in the typical "woundhealing" experiment presumably results because the migrating cells have an increased ability to use serum factors. Serum-factor requirements for "wound healing" in cultured 3T3 cells are discussed.
摘要
一种促进（小鼠）3T3成纤维细胞迁移的血清因子被证明与血清中的生长促进因子和细胞存活因子不同。该因子促进汇合的3T3细胞中伤口边缘细胞的迁移，但在这些条件下细胞迁移不会引发DNA合成。随后添加血清会引发迁移细胞中的DNA合成。结果表明，静止的3T3细胞之间的接触中断不足以引发DNA合成。在典型的“伤口愈合”实验中，迁移的3T3细胞中观察到的DNA合成可能是因为迁移细胞利用血清因子的能力增强。讨论了培养的3T3细胞“伤口愈合”对血清因子的需求。
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