Dulbecco R, Stoker M G
Proc Natl Acad Sci U S A. 1970 May;66(1):204-10. doi: 10.1073/pnas.66.1.204.
Experiments were designed to discriminate between inhibition of growth due to contacts or exhaustion of serum factors. The cell layer was wounded and the migrating cells were followed by time-lapse cinematography; DNA synthesis in the same cells was recognized by means of (3)H-thymidine labeling and radioautography. In this way, the complete history of individual cells migrating to the wound could be described. The results show that topographical relationships between cells play an important role in controlling initiation of DNA synthesis. It is still unclear whether initiation is promoted by release from contacts or by the increased ability of the cells to utilize serum factors because of their changes in shapes and activities.
实验旨在区分因细胞接触或血清因子耗尽导致的生长抑制。对细胞层造成创伤，通过延时摄影追踪迁移的细胞；利用³H-胸腺嘧啶核苷标记和放射自显影识别同一细胞中的DNA合成。通过这种方式，可以描述单个细胞迁移至伤口处的完整过程。结果表明，细胞间的拓扑关系在控制DNA合成起始方面发挥着重要作用。目前尚不清楚起始是由接触解除所促进，还是由于细胞形状和活性的改变使其利用血清因子的能力增强所导致。
