Hatanaka M
Proc Natl Acad Sci U S A. 1973 May;70(5):1364-7. doi: 10.1073/pnas.70.5.1364.
Transformation of NIH Swiss mouse fibroblasts by a murine sarcoma virus was influenced by the type of carbohydrate in the culture medium. Exposure to D-mannose and 2-deoxy-D-glucose caused a reduction in the number of transformed colonies in vitro and tumor formation in vivo. This is a new type of interference in the transformation process, manifested also by the absence of the pronounced enhancement of hexose entry into transformed cells, characteristic of a sarcoma virus infection. The uptake of D-glucose, D-mannose, and D-galactose, was repressed by normal mouse cells grown in the presence of D-mannose and D-glucose in the medium, while uptake of the same sugars was derepressed in cells grown in media containing D-xylose, L-xylose, or L-arabinose. D-Galactose had an intermediate effect on hexose uptake and permitted derepression. In every case, enhancement of the primary transport by sarcoma virus transformation was consistently beyond that observed at any time in the derepressed control cells, demonstrating the occurrence of another mechanism of enhancement of hexose uptake after sarcoma virus infection.
鼠肉瘤病毒对NIH瑞士小鼠成纤维细胞的转化受培养基中碳水化合物类型的影响。暴露于D-甘露糖和2-脱氧-D-葡萄糖会导致体外转化集落数量减少以及体内肿瘤形成。这是转化过程中一种新型的干扰，其表现还包括缺乏肉瘤病毒感染所特有的己糖进入转化细胞的明显增强。培养基中存在D-甘露糖和D-葡萄糖时生长的正常小鼠细胞会抑制D-葡萄糖、D-甘露糖和D-半乳糖的摄取，而在含有D-木糖、L-木糖或L-阿拉伯糖的培养基中生长的细胞中，相同糖类的摄取则会解除抑制。D-半乳糖对己糖摄取有中间作用并允许解除抑制。在每种情况下，肉瘤病毒转化导致的初级转运增强始终超过在解除抑制的对照细胞中任何时候观察到的增强，这表明肉瘤病毒感染后存在另一种己糖摄取增强机制。
