肝脏色氨酸加氧酶信使核糖核酸：其部分纯化、在异源无细胞系统中的翻译以及糖皮质激素对其的调控。
Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.
作者信息
Schutz G, Beato M, Feigelson P
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1218-21. doi: 10.1073/pnas.70.4.1218.
Abstract
Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenger RNA directs the initiation, synthesis, and release of a product that was identified as complete subunits of hepatic tryptophan oxygenase (EC 1.13.1.12) by immunoprecipitation with monovalent antibodies prepared against homogeneous tryptophan oxygenase and subsequent sodium dodecyl sulfate-polyacrylamide electrophoresis of the solubilized immunoprecipitate. This may represent the first complete translation in a heterologous system of a mammalian messenger RNA coding for an enzyme protein. Analysis of the messenger RNA content of the liver after glucocorticoid administration demonstrates that the hormonally enhanced rate of synthesis of tryptophan oxygenase is accompanied by an increased quantity of its corresponding messenger RNA.
摘要
大鼠肝脏的信使核糖核酸（mRNA）基于其聚腺苷酸（poly(A)）含量，通过在纤维素上进行层析得以部分纯化。微克量的这种RNA能在源自克雷布斯腹水细胞并补充网织红细胞起始因子的异源无细胞体系中，将蛋白质合成刺激许多倍。该信使核糖核酸指导一种产物的起始、合成及释放，通过用针对纯一的色氨酸加氧酶制备的单价抗体进行免疫沉淀，以及随后对溶解的免疫沉淀物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，该产物被鉴定为肝色氨酸加氧酶（EC 1.13.1.12）的完整亚基。这可能代表了在异源体系中首次对编码一种酶蛋白的哺乳动物信使核糖核酸进行完整翻译。对给予糖皮质激素后肝脏信使核糖核酸含量的分析表明，色氨酸加氧酶合成速率的激素增强伴随着其相应信使核糖核酸数量的增加。
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Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.肝脏色氨酸加氧酶信使核糖核酸：其部分纯化、在异源无细胞系统中的翻译以及糖皮质激素对其的调控。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1218-21. doi: 10.1073/pnas.70.4.1218.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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THE ROLES OF SYNTHESIS AND DEGRADATION IN THE CONTROL OF RAT LIVER TRYPTOPHAN PYRROLASE.合成与降解在大鼠肝脏色氨酸吡咯酶调控中的作用
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STIMULATION OF MESSENGER RNA SYNTHESIS IN RAT LIVER BY CORTISOL.皮质醇对大鼠肝脏信使核糖核酸合成的刺激作用
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Studies on the mechanism of regulation by cortisone of the metabolism of liver purine and ribonucleic acid.可的松对肝脏嘌呤和核糖核酸代谢调节机制的研究。
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Identification of free cytoplasmic globin mRNA of duck erythroblasts by hybridization to anti-messenger DNA and by cell-free protein synthesis.通过与抗信使DNA杂交及无细胞蛋白质合成鉴定鸭成红细胞的游离细胞质珠蛋白mRNA
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The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA.在用小鼠网织红细胞9S RNA编程的兔网织红细胞无细胞体系中合成小鼠血红蛋白β链。
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Synthesis of myosin on heterologous ribosomes.肌球蛋白在异源核糖体上的合成。
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Synthesis of ovalbumin in a rabbit reticulocyte cell-free system programmed with hen oviduct ribonucleic acid.用鸡输卵管核糖核酸编程的兔网织红细胞无细胞系统中卵清蛋白的合成。
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