Shinomiya T, Scherer G, Schmid W, Zentgraf H, Schütz G
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1346-50. doi: 10.1073/pnas.81.5.1346.
Tyrosine aminotransferase (TAT; L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5) from rat liver is subject to glucocorticoid, cAMP, and developmental control. To study the underlying regulatory mechanisms, the TAT structural gene was isolated from a lambda bacteriophage rat DNA library. Heteroduplex analysis revealed that the 2.4-kilobase-long TAT mRNA is encoded by a gene that extends over 11 kilobases and is interrupted by 11 introns. To characterize the presumptive control region, the DNA sequence around the 5' end of the gene was determined and the start site of transcription was identified by nuclease S1 protection experiments. A short sequence homology in an equivalent position relative to the cap site was detected between TAT and tryptophan oxygenase, another glucocorticoid-controlled gene from rat liver. This sequence is related to the sequence 5' T-G-T-T-C-T 3' found in regions of the long terminal repeat of mouse mammary tumor virus, which has been shown to interact with the glucocorticoid receptor [Scheidereit, C., Geisse, J., Westphal, H. M. & Beato, M. (1983) Nature (London) 304, 749-752].
大鼠肝脏中的酪氨酸转氨酶（TAT；L-酪氨酸：2-氧代戊二酸转氨酶，EC 2.6.1.5）受糖皮质激素、环磷酸腺苷（cAMP）和发育调控。为了研究其潜在的调控机制，从λ噬菌体大鼠DNA文库中分离出TAT结构基因。异源双链分析表明，长度为2.4千碱基的TAT mRNA由一个跨越11千碱基且被11个内含子打断的基因编码。为了表征假定的调控区域，测定了该基因5'端周围的DNA序列，并通过核酸酶S1保护实验确定了转录起始位点。在TAT与色氨酸加氧酶（大鼠肝脏中另一个受糖皮质激素调控的基因）相对于帽位点的等效位置检测到一个短序列同源性。该序列与在小鼠乳腺肿瘤病毒长末端重复序列区域中发现的5'T-G-T-T-C-T 3'序列相关，已证明该序列与糖皮质激素受体相互作用[谢德赖特，C.，盖斯，J.，韦斯特法尔，H.M. & 贝托，M.（1983年）《自然》（伦敦）304，749 - 752]。
