Glick M C, Kimhi Y, Littauer U Z
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1682-7. doi: 10.1073/pnas.70.6.1682.
Sequential removal of surface glycopeptides was achieved by subjection of mouse neuroblastoma cells to a two-step trypsin treatment under different conditions. The glycopetides released by each trypsinization step were digested by Pronase and examined on columns of Sephadex G-50. Different chromatographic patterns were found for the two digests. Thus, several groups of glycopeptides can be distinguished by the trypsinization procedure. One group is readily removed and appears to be at a more accessible location on the cell surface. Among the four neuroblastoma clones examined, the glycopeptide patterns from axon-forming cells differed from those of axon-minus cells.
通过在不同条件下对小鼠神经母细胞瘤细胞进行两步胰蛋白酶处理，实现了表面糖肽的顺序去除。每个胰蛋白酶消化步骤释放的糖肽用链霉蛋白酶消化，并在Sephadex G - 50柱上进行检测。两种消化产物呈现出不同的色谱模式。因此，通过胰蛋白酶消化程序可以区分几组糖肽。一组很容易被去除，似乎位于细胞表面更容易接近的位置。在所检测的四个神经母细胞瘤克隆中，形成轴突的细胞的糖肽模式与无轴突细胞的不同。
