通过添加L-抗坏血酸加速甘氨酰精氨酰天冬氨酰精氨酰甘氨酸和人转铁蛋白的脱酰胺化速率。
Acceleration of the rate of deamidation of GlyArgAsnArgGly and of human transferrin by addition of L-ascorbic acid.
作者信息
Robinson A B, Irving K, McCrea M
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2122-3. doi: 10.1073/pnas.70.7.2122.
Abstract
Experiments on the model peptide, GlyArgAsnArgGly, and the protein, human transferrin, have shown that hydrolytic deamidation of these molecules is markedly accelerated by addition of physiologically significant concentrations of l-ascorbic acid. Since hydrolytic demidation has been suggested as an important timer of biological events, the effects on hydrolytic deamidation of substances that are normally present in living organisms and are subject to nutritional control are of special relevance.
摘要
对模型肽GlyArgAsnArgGly和蛋白质人转铁蛋白进行的实验表明，添加生理浓度的L-抗坏血酸可显著加速这些分子的水解脱酰胺作用。由于水解脱酰胺作用被认为是生物事件的重要计时机制，因此研究活生物体中正常存在且受营养控制的物质对水解脱酰胺作用的影响具有特殊意义。
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