激光拉曼光谱研究胆固醇对二棕榈酰卵磷脂多层膜构象变化的影响。
Laser Raman investigation of the effect of cholesterol on conformational changes in dipalmitoyl lecithin multilayers.
作者信息
Lippert J L, Peticolas W L
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1572-6. doi: 10.1073/pnas.68.7.1572.
Abstract
Large and abrupt changes are observed at 38 degrees C in the 1100 cm(-1) region of the Raman spectrum of aqueous dipalmitoyl lecithin multilayers. They correspond to conformational changes due to the melting of the paraffin side chains. The addition of cholesterol to the multilayers broadens but does not abolish these changes. It is suggested that the addition of cholesterol decreases the interactions between adjacent paraffin side chains of lecithin, causing a change from a cooperative to a noncooperative gel-liquid crystal transition. Removal of water from dipalmitoyl lecithin also results in a noncooperative transition strikingly similar to that caused by addition of cholesterol. Raman spectroscopy thus provides a new and sensitive probe for analyzing the structures of membranes and their constituents.
摘要
在38摄氏度时，观察到水合二棕榈酰卵磷脂多层膜的拉曼光谱在1100厘米-1区域有大幅度的突然变化。这些变化对应于由于石蜡侧链熔化引起的构象变化。向多层膜中添加胆固醇会使这些变化变宽，但不会消除这些变化。有人认为，添加胆固醇会减少卵磷脂相邻石蜡侧链之间的相互作用，导致从协同的凝胶-液晶转变变为非协同的转变。从二棕榈酰卵磷脂中除去水也会导致与添加胆固醇引起的转变极为相似的非协同转变。因此，拉曼光谱为分析膜及其成分的结构提供了一种新的灵敏探针。
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