Wisnieski B J, Williams R E, Fox C F
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3669-73. doi: 10.1073/pnas.70.12.3669.
The fatty acid composition of animal cells cultured in serum-free medium can be manipulated when the synthesis of endogenous fatty acids is inhibited by a biotin analog and fatty acids are supplied in the medium as detergent esters of Tween. When mouse LM cells were grown in medium supplemented with Tween-19:0 (an ester of Tween and nonadecanoic acid), odd chain fatty acid content of cellular phospholipids and neutral lipids increased from 1% to 75%. Concurrently, the saturated fatty acid content increased from 27% to 85%. Similar alterations in fatty acid content have been observed when BHK(21) cells are subjected to the same enrichment regime. The ability to control the fatty acid composition of cultured animal cells is a prerequisite to investigations into the role of the membrane lipid physical state in processes unique to these cells.
当内源性脂肪酸的合成被生物素类似物抑制，且脂肪酸以吐温的去污剂酯形式添加到无血清培养基中时，在这种培养基中培养的动物细胞的脂肪酸组成可以被调控。当小鼠LM细胞在补充了吐温-19:0（吐温和十九烷酸的酯）的培养基中生长时，细胞磷脂和中性脂质中的奇数链脂肪酸含量从1%增加到了75%。同时，饱和脂肪酸含量从27%增加到了85%。当BHK(21)细胞采用相同的富集方案时，也观察到了脂肪酸含量的类似变化。控制培养动物细胞的脂肪酸组成的能力，是研究膜脂物理状态在这些细胞特有过程中的作用的一个先决条件。
