Holley R W, Baldwin J H, Kiernan J A
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3976-8. doi: 10.1073/pnas.71.10.3976.
The growth of mouse myeloma XS 63.5 cells in cell culture is dependent on serum. Among the several growth factors present in serum, the lipid fraction is highly active. The growth factor(s) provided by the serum lipid fraction can be replaced by unsaturated fatty acids. If XS 63.5 cells are cultured in medium that is low in serum lipids, the cells become quiescent, with most of the cells in the G(1) (or G(0)) phase of the cell cycle. Addition of linoleic acid to such quiescent cells leads to reinitiation of DNA synthesis and growth.
小鼠骨髓瘤XS 63.5细胞在细胞培养中的生长依赖于血清。血清中存在的几种生长因子中,脂质部分具有高度活性。血清脂质部分提供的生长因子可被不饱和脂肪酸替代。如果将XS 63.5细胞培养在血清脂质含量低的培养基中,细胞会进入静止状态,大多数细胞处于细胞周期的G(1)(或G(0))期。向此类静止细胞中添加亚油酸会导致DNA合成和生长重新开始。