噬菌体λ的整合组成型突变体。
Int-constitutive mutants of bacteriophage lambda.
作者信息
Shimada K, Campbell A
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):237-41. doi: 10.1073/pnas.71.1.237.
Abstract
The constitutive production of small amounts of trpB enzyme in an Escherichia coli strain carrying lambdacI857 prophage within the trpC gene has been examined in derivatives of this strain from which portions of the prophage have been deleted. Enzyme production requires a site (p(I)) within the prophage close to the left prophage end. Selection for mutants of this lysogen that grow on low concentrations of indole yielded two types of mutations within the prophage: (a) v2-type, in which all phage genes controlled by the major leftward operator are derepressed; and (b) int-c type, in which the only phage gene derepressed is int. The int-c mutations lie in the same part of the prophage as p(I). All int-c mutants appear deficient in xis gene function, even when derepressed.
摘要
在trpC基因内携带λcI857原噬菌体的大肠杆菌菌株中，已对该菌株的衍生物进行了研究，这些衍生物中部分原噬菌体已被删除，以此来检测少量trpB酶的组成型产生。酶的产生需要原噬菌体中靠近原噬菌体左端的一个位点（p(I)）。对在低浓度吲哚上生长的这种溶原菌的突变体进行筛选，在原噬菌体中产生了两种类型的突变：(a) v2型，其中由主要向左操纵子控制的所有噬菌体基因均被去阻遏；(b) int-c型，其中唯一被去阻遏的噬菌体基因是int。int-c突变位于原噬菌体中与p(I)相同的部分。所有int-c突变体即使在去阻遏时，xis基因功能似乎也存在缺陷。
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