噬菌体λ的N基因产物诱导大肠杆菌中σ因子的合成。
Induction of sigma factor synthesis in Escherichia coli by the N gene product of bacteriophage lambda.
作者信息
Nakamura Y, Yura T
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4405-9. doi: 10.1073/pnas.73.12.4405.
Abstract
Thermoinduction of cells of E. coli carrying prophage lambdacI857 within the bfe gene brings about not only "escape synthesis" of core subunits of the DNA-dependent RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2-7-7-6), but also a striking stimulation of sigma factor synthesis. The latter phenomenon, termed sigma induction, is generally observed after lambda phage infection or prophage induction. A series of experiments with various bacterial and phage strains led us to conclude that the N gene product of lambda is directly involved to the sigma induction. These and other results obtained with mutants defective in transcription termination factor rho suggest the involvement of a rho-sensitive site in the control of sigma gene expression in E. coli.
摘要
携带位于bfe基因内的原噬菌体λcI857的大肠杆菌细胞经热诱导后，不仅会导致依赖DNA的RNA聚合酶（RNA核苷酸转移酶，核苷三磷酸：RNA核苷酸转移酶，EC 2-7-7-6）核心亚基的“逃逸合成”，还会显著刺激σ因子的合成。后一种现象，即σ诱导，通常在λ噬菌体感染或原噬菌体诱导后观察到。一系列针对各种细菌和噬菌体菌株的实验使我们得出结论，λ的N基因产物直接参与了σ诱导。这些以及用转录终止因子ρ缺陷的突变体获得的其他结果表明，一个对ρ敏感的位点参与了大肠杆菌中σ基因表达的调控。
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