Crandall M, Lawrence L M, Saunders R M
Proc Natl Acad Sci U S A. 1974 Jan;71(1):26-9. doi: 10.1073/pnas.71.1.26.
Cell fusion between opposite mating types 5 and 21 of the yeast Hansenula wingei is initiated by a strong sexual agglutination reaction. The mating factors responsible for the specificity of cellular recognition are complementary glycoproteins which form a physical complex in vitro. The complex is assayed by recovery of agglutination activity of the multivalent 5-factor after the univalent 21-factor has been inactivated by treatment of the complex with alkali. The 5-factor.21-factor complex, purified on Sepharose 6B, is large (several million daltons) and heterogeneous. The three peaks of 5-factor activity contain a number of combining sites proportional to molecular size.
汉逊酵母5型和21型相反交配型之间的细胞融合是由强烈的有性凝集反应引发的。负责细胞识别特异性的交配因子是互补糖蛋白,它们在体外形成物理复合物。通过用碱处理复合物使单价21因子失活后,通过恢复多价5因子的凝集活性来检测该复合物。在琼脂糖6B上纯化的5因子-21因子复合物很大(数百万道尔顿)且不均一。5因子活性的三个峰含有与分子大小成比例的多个结合位点。