莱茵衣藻的配子分化。II. 鞭毛膜与凝集反应。
Gametic differentiation in Chlamydomonas reinhardtii. II. Flagellar membranes and the agglutination reaction.
作者信息
Bergman K, Goodenough U W, Goodenough D A, Jawitz J, Martin H
出版信息
J Cell Biol. 1975 Dec;67(3):606-22. doi: 10.1083/jcb.67.3.606.
Abstract
A structural and biochemical study is presented concerning the agglutination of gametic flagella, the initial step in the mating reaction of Chlamydomonas reinhardtii. An alteration in the distribution of the intramembranous particles revealed by freeze-fracturing of flagella membranes is shown to accompany gametic differentiation in both mating types. The isolation and electrophoretic analysis of flagellar membranes and mastigonemes are reported; no electrophoretic differences can be detected when the membrane or mastigoneme glycoproteins from vegative and gametic cells are compared, nor when glycoproteins from the two mating types are compared, and no novel polypeptides are present in gametic preparations. The membrane vesicles, after they are freed of mastigonemes by sedimentation through a discontinuous sucrose gradient, are extremely active as an isoagglutinin, indicating a direct involvement of the membrane in the mating reaction.
摘要
本文介绍了一项关于莱茵衣藻交配反应初始步骤——配子鞭毛凝集的结构和生化研究。通过鞭毛膜冷冻断裂揭示的膜内颗粒分布变化表明，两种交配类型的配子分化过程中均会出现这种变化。报告了鞭毛膜和鞭毛茸的分离及电泳分析结果；比较营养细胞和配子细胞的膜或鞭毛茸糖蛋白时，以及比较两种交配类型的糖蛋白时，均未检测到电泳差异，并且配子制剂中也不存在新的多肽。通过不连续蔗糖梯度沉降去除鞭毛茸后的膜泡作为同种凝集素具有极高活性，这表明膜直接参与了交配反应。
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