抗体多样性的种系基础：通过DNA-RNA杂交测量免疫球蛋白VH和CH基因频率
Germ line basis for antibody diversity: immunoglobulin VH-and CH-gene frequencies measured by DNA-RNA hybridization.
作者信息
Premkumar E, Shoyab M, Williamson A R
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):99-103. doi: 10.1073/pnas.71.1.99.
Abstract
The reiteration frequency for mouse immunoglobulin V(H)-genes and C(H)-genes has been directly estimated by hybridization of purified MOPC 315 alpha-chain mRNA with a vast excess of mouse DNA. A biphasic C(o)t curve resulted. The low C(o)t transition (C(o)t(1/2) about 1.5) was interpreted as hybridization to V(H)-genes and the high C(o)t transition (C(o)t(1/2) about 10(3)) as hybridization to C(H)-genes. These values correspond to about 5000 V(H)-genes and less than 8 C(H)-genes. This germ-line content of V(H)-genes is sufficient to account for antibody diversity given a comparable set of V(L)-genes. Minimal-gene models are invalidated and there is no need to invoke somatic generators of diversity.
摘要
通过将纯化的MOPC 315α链mRNA与大量过量的小鼠DNA进行杂交，直接估算了小鼠免疫球蛋白V(H)基因和C(H)基因的重复频率。得到了一条双相Cot曲线。低Cot转变（Cot(1/2)约为1.5）被解释为与V(H)基因杂交，高Cot转变（Cot(1/2)约为10³）被解释为与C(H)基因杂交。这些值分别对应约5000个V(H)基因和少于8个C(H)基因。在有一组相当的V(L)基因的情况下，这种V(H)基因的种系含量足以解释抗体多样性。最小基因模型被推翻，无需引入体细胞多样性产生机制。
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