来自磷酸胆碱结合蛋白的免疫球蛋白重链之间的大小差异。
Size differences among immunoglobulin heavy chains from phosphorylcholine-binding proteins.
作者信息
Rudikoff S, Potter M
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2109-12. doi: 10.1073/pnas.73.6.2109.
Abstract
The entire sequences of the heavy chain variable regions of M167 and TEPC 15 (phosphorylcholine-binding myeloma proteins of BALB/c origin) have been determined. These sequences are compared with the phosphorylcholine-binding protein M603. T15 differs from M603 at four positions, all of which are located in antigen-binding complementarity regions. M167, in addition to having differences in the complementarity regions, also has five substitutions in the conserved framework portion of the variable region when compared to T15 and M603. Each of the three proteins has a different length in the third complementarity region. It is unlikely that complementarity regions of different lengths associated with similar framework regions could be generated by proposed mechanisms of somatic mutation which are generally limited to point mutations. It appears more likely that these products are directly encoded by different structural germ line genes.
摘要
已确定M167和TEPC 15（源自BALB/c的磷酸胆碱结合骨髓瘤蛋白）重链可变区的完整序列。将这些序列与磷酸胆碱结合蛋白M603进行比较。T15在四个位置与M603不同，所有这些位置都位于抗原结合互补区。与T15和M603相比，M167除了在互补区存在差异外，其可变区的保守框架部分还存在五个替换。这三种蛋白质在第三个互补区的长度各不相同。与相似框架区相关的不同长度的互补区不太可能由通常限于点突变的体细胞突变的提出机制产生。这些产物似乎更有可能由不同的结构种系基因直接编码。
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