含有Hg2+和Ag+的Cs2S04梯度能实现中性CsCl梯度中密度相同的卫星脱氧核糖核酸的完全分离。
Cs2S04 gradients containing both Hg2+ and Ag+ effect the complete separation of satellite deoxyribonucleic acids having identical densities in neutral CsCl gradients.
作者信息
Skinner D M, Beattie W G
出版信息
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3108-10. doi: 10.1073/pnas.70.11.3108.
Abstract
A combination of both Ag(+) and Hg(2+) in Cs(2)SO(4) effects the complete separation of two DNAs having identical densities in CsCl. Satellite DNAs of hermit crab, Pagurus pollicaris, and lobster, Homarus americanus, have been isolated by this means.
摘要
Cs₂SO₄ 中 Ag⁺ 和 Hg²⁺ 的组合能够实现 CsCl 中具有相同密度的两种 DNA 的完全分离。通过这种方法已分离出海蟹 Pagurus pollicaris 和龙虾 Homarus americanus 的卫星 DNA。
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Proc Natl Acad Sci U S A. 1967 Jul;58(1):103-10. doi: 10.1073/pnas.58.1.103.
2
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Proc Natl Acad Sci U S A. 1965 Jan;53(1):111-8. doi: 10.1073/pnas.53.1.111.
3
The reversible reaction of Hg (II) and double-stranded polynucleotides. A step-function theory and its significance.汞（II）与双链多核苷酸的可逆反应。阶跃函数理论及其意义。
Biochim Biophys Acta. 1963 Feb 26;68:240-53. doi: 10.1016/0006-3002(63)90139-2.
4
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6
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7
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