Slater I, Slater D W
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1103-7. doi: 10.1073/pnas.71.4.1103.
Polyadenylylated RNA from sea urchin embryos concomitantly labeled with [(3)H]adenosine and [(14)C]uridine between fertilization and the four-cell stage was used to determine whether the RNA primers prerequisite to the massive polyadenylylation known to occur after fertilization are synthesized during oogenesis or subsequent to fertilization. Characterization of this RNA and unlabeled RNA via retention on nitrocellulose membranes and poly(U)-impregnated filters, molecular hybridization with [(3)H]poly(U), RNase resistance, oligo(dT)-cellulose chromatography, and size-distribution studies indicates that the poly(A) tracts synthesized after fertilization are predominantly appended to preexisting cytoplasmic primers of oogenic origin. Hence, if polyadenylylation is involved in the selective editing of presumptive genetic messages, this process is not confined to the nucleus unless a given codogenic transcript can undergo more than one cycle of adenylylation.
用在受精至四细胞期之间同时用[³H]腺苷和[¹⁴C]尿苷标记的海胆胚胎的聚腺苷酸化RNA，来确定受精后已知发生的大量聚腺苷酸化所必需的RNA引物是在卵子发生过程中合成的，还是在受精后合成的。通过硝酸纤维素膜和聚（U）浸渍滤膜上的保留、与[³H]聚（U）的分子杂交、核糖核酸酶抗性、寡聚（dT）-纤维素色谱分析以及大小分布研究对该RNA和未标记RNA进行表征，结果表明受精后合成的聚（A）链主要附加到卵子发生来源的预先存在的细胞质引物上。因此，如果聚腺苷酸化参与了推定遗传信息的选择性编辑，那么这个过程并不局限于细胞核，除非给定的编码转录本可以经历不止一个腺苷酸化循环。
