一种参与噬菌体φX174颗粒形成的DNA-蛋白质复合物。
A DNA-protein complex involved in bacteriophage phi chi 174 particle formation.
作者信息
Weisbeek P J, Sinsheimer R L
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3054-8. doi: 10.1073/pnas.71.8.3054.
Abstract
A varphiX-specific DNA-protein complex has been isolated from varphiX-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.
摘要
已从感染φX的细胞中分离出一种特异性的φX DNA-蛋白质复合物。该复合物含有感染性环状单链DNA、基因F、G、H和J的蛋白质，其比例与噬菌体颗粒中的相同，此外还含有基因D-蛋白质。D-蛋白质占复合物蛋白质部分的12%-22%。该复合物的沉降值约为140S。在体外，该复合物可转化为正常的114S噬菌体颗粒（同时其D-蛋白质会丢失），或转化为无感染性的70S颗粒和少量游离单链DNA。快速沉降颗粒与膜无关。
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