在噬菌体φX174组装过程中分离出的病毒DNA合成中间复合物。
Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.
作者信息
Fujisawa H, Hayashi M
出版信息
J Virol. 1976 Aug;19(2):409-15. doi: 10.1128/JVI.19.2.409-415.1976.
Abstract
A DNA protein complex that is a precursor of mature phi X174 phage was isolated. The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA. The viral-strand DNA ranged from one to two genomes in length. Proteins coded on the phi X174 genome as well as the host genome were associated with the viral DNA in the 50S precursor complex. Our results indicated that both viral DNA synthesis and cleavage of the growing viral-strand DNA occurred in the 50S complex.
摘要
一种作为成熟φX174噬菌体前体的DNA-蛋白质复合物被分离出来。该复合物在蔗糖梯度中的沉降系数S为50，包含由具有单链病毒DNA延伸尾的复制形式分子组成的噬菌体DNA。病毒链DNA的长度为1到2个基因组。φX174基因组以及宿主基因组编码的蛋白质与50S前体复合物中的病毒DNA相关联。我们的结果表明，病毒DNA合成和正在生长的病毒链DNA的切割都发生在50S复合物中。
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