噬菌体φX174的基因A产物是体内单链DNA合成过程中位点特异性内切核酸酶切割所必需的。
Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo.
作者信息
Fujisawa H, Hayashi M
出版信息
J Virol. 1976 Aug;19(2):416-24. doi: 10.1128/JVI.19.2.416-424.1976.
Abstract
A functional gene A product of phi X174 was found to be required at the stage of single-stranded DNA synthesis. A precursor complex that synthesizes single-stranded DNA (50S complex [Fujisawa and Hayashi, 1976]) was isolated from cells infected with wild-type or with temperature-sensitive gene A mutant phage. Proper cleavage of the single-stranded viral DNA did not occur in cells infected with the temperature-sensitive gene A mutant under restrictive conditions. This resulted in (i) accumulation of linear viral DNA molecules of 2 units in length in the 50S complex and (ii) cessation of elongation of viral-strand DNA after one complete cycle of single-stranded DNA synthesis.
摘要
发现φX174的功能性基因A产物在单链DNA合成阶段是必需的。从感染野生型或温度敏感型基因A突变噬菌体的细胞中分离出了合成单链DNA的前体复合物（50S复合物[藤泽和林，1976]）。在限制条件下，感染温度敏感型基因A突变体的细胞中，单链病毒DNA未发生正确切割。这导致（i）50S复合物中积累长度为2个单位的线性病毒DNA分子，以及（ii）单链DNA合成一个完整循环后病毒链DNA延伸停止。
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引用本文的文献
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Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.由嗜沫嗜血杆菌和流感嗜血杆菌H-I的限制酶产生的φX-174复制性DNA的片段图谱。
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