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细胞显微荧光测定法:一种快速荧光测量方法。

Cell microfluorometry: a method for rapid fluorescence measurement.

作者信息

Van Dilla M A, Trujillo T T, Mullaney P F, Coulter J R

出版信息

Science. 1969 Mar 14;163(3872):1213-4. doi: 10.1126/science.163.3872.1213.

Abstract

A high-speed flow system for quantitative determination of fluoresence of cells containing fluorochrome has been developed. Feulgen-DNA distributions in populations of tissue culture cells and human leukocytes havebeen measured at a rate of 10(4) to 10(5) cells per minute and compare well with results of other independent methods.

摘要

已开发出一种用于定量测定含荧光染料细胞荧光的高速流动系统。已以每分钟10⁴至10⁵个细胞的速率测量了组织培养细胞群体和人类白细胞中的福尔根 - DNA分布,并且与其他独立方法的结果相当吻合。

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