干扰素对弗氏白血病细胞的作用。I. 未处理及经二甲基亚砜处理的细胞中病毒和红系标志物的表达

Interferon effects on Friend leukaemia cells. I. EXpression of virus and erythroid markers in untreated and dimethyl sulphoxide-treated cells.

作者信息

Dolei A, Colletta G, Capobianchi M R, Rossi G B, Vecchio G

出版信息

J Gen Virol. 1980 Jan;46(1):227-36. doi: 10.1099/0022-1317-46-1-227.

Abstract

The effects of low doses (40 to 1000 units/ml) of mouse interferon (IF) on the expression of Friend leukaemia virus (FLV) and globin genes in Friend leukaemia cells (FLC) have been examined. IF blocks production of extracellular virus, but virus antigens accumulate in the cytoplasm. In cells treated with IF at the time of seeding, there is a reduction in the amount of RNA specified by the lymphatic leukaemia virus (LLV) component of FLV; with the same IF dose there is a small but definite stimulation of haemoglobin and globin mRNA synthesis. The effects of IF on LLV gene expression are even more pronounced in dimethyl sulphoxide (DMSO)-stimulated LFC. No correlation was found between LLV gene expression and the appearance of erythroid markers.

摘要

研究了低剂量（40至1000单位/毫升）小鼠干扰素（IF）对Friend白血病细胞（FLC）中Friend白血病病毒（FLV）和珠蛋白基因表达的影响。IF可阻断细胞外病毒的产生，但病毒抗原会在细胞质中积累。在接种时用IF处理的细胞中，FLV的淋巴白血病病毒（LLV）成分所指定的RNA量减少；在相同的IF剂量下，血红蛋白和珠蛋白mRNA的合成有轻微但确定的刺激作用。在二甲基亚砜（DMSO）刺激的LFC中，IF对LLV基因表达的影响更为明显。未发现LLV基因表达与红系标志物的出现之间存在相关性。