Melchers F, Lernhardt W
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7681-5. doi: 10.1073/pnas.82.22.7681.
The cell cycle of activated B lymphocytes was found to be controlled by three restriction points. The first occurs immediately after mitosis and was found to be controlled by the binding of Sepharose-bound, immunoglobulin-specific antibodies to surface membrane-bound immunoglobulin. Exposure to this stimulus as short as 15 min or as long as 36 hr allowed B cells to move into the G1 phase up to the next restriction point. The second restriction point was observed to be approximately equal to 4 hr after mitosis, in the G1 phase of the cycle and 3-4 hr before the B cells entered S phase, and was found to be controlled by alpha-type B-cell growth factors produced by the P388D1 macrophage line. A third restriction point occurs in the G2 phase, 2-4 hr before mitosis, and is apparently controlled by beta-type B-cell growth factors that are likely to be produced by helper T lymphocytes.
已发现活化B淋巴细胞的细胞周期受三个限制点控制。第一个限制点在有丝分裂后立即出现，发现它受琼脂糖结合的免疫球蛋白特异性抗体与表面膜结合免疫球蛋白的结合所控制。暴露于这种刺激15分钟或长达36小时，可使B细胞进入G1期直至下一个限制点。第二个限制点在有丝分裂后约4小时观察到，处于细胞周期的G1期，在B细胞进入S期前3 - 4小时，发现它受P388D1巨噬细胞系产生的α型B细胞生长因子控制。第三个限制点出现在G2期，有丝分裂前2 - 4小时，显然受可能由辅助性T淋巴细胞产生的β型B细胞生长因子控制。
