纤维蛋白原的构象：三结节结构的量热学证据
Conformation of fibrinogen: calorimetric evidence for a three-nodular structure.
作者信息
Donovan J W, Mihalyi E
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4125-8. doi: 10.1073/pnas.71.10.4125.
Abstract
Solutions of fibrinogen show two endothermal (denaturing) transitions, at 61 degrees and at 100 degrees , when heated in a differential scanning calorimeter. Similar transitions are observed for a mixture of the fragments D and E obtained by limited proteolysis of fibrinogen. Isolated fragment E shows only a single transition, at 97 degrees . The independent thermal denaturation of these portions of fibrinogen supports the three-nodular model proposed for fibrinogen. The D and E subunits retain their characteristic denaturation behavior when fibrinogen is clotted by thrombin addition, but over a period of about one hundred times the clotting time, the denaturation temperature of the D subunit increases by 9 degrees and its enthalpy of denaturation by one-third. Since this change takes place in the absence of Factor XIII activity, and its rate is proportional to thrombin concentration, it is presumed to be mediated by a proteolytic cleavage distinct from those which liberate the A and B fibrinopeptides.
摘要
在差示扫描量热仪中加热时，纤维蛋白原溶液在61度和100度出现两个吸热（变性）转变。对通过纤维蛋白原有限蛋白酶解获得的片段D和E的混合物也观察到类似的转变。分离出的片段E仅在97度出现单一转变。纤维蛋白原这些部分的独立热变性支持了为纤维蛋白原提出的三结节模型。当通过添加凝血酶使纤维蛋白原凝结时，D和E亚基保留其特征性变性行为，但在约凝血时间的一百倍时间内，D亚基的变性温度升高9度，其变性焓增加三分之一。由于这种变化在缺乏因子XIII活性的情况下发生，且其速率与凝血酶浓度成正比，推测它是由不同于释放A和B纤维蛋白肽的蛋白水解切割介导的。
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本文引用的文献
1
The polymerization of proteins; the action of thrombin on fibrinogen.蛋白质的聚合；凝血酶对纤维蛋白原的作用。
Arch Biochem Biophys. 1951 Jul;32(2):317-24. doi: 10.1016/0003-9861(51)90277-9.
2
Digestion of fibrinogen by trypsin. II. Characterization of the large fragment obtained.
Biochim Biophys Acta. 1963 Jan 8;67:90-103. doi: 10.1016/0006-3002(63)91799-2.
3
Digestion of fibrinogen by trypsin. I. Kinetic studies of the reaction.胰蛋白酶对纤维蛋白原的消化作用。I. 反应的动力学研究。
Biochim Biophys Acta. 1963 Jan 8;67:73-89. doi: 10.1016/0006-3002(63)91798-0.
4
[The products of degradation of human fibrinogen by plasmin. I. Separation and physicochemical properties].[纤溶酶对人纤维蛋白原的降解产物。I. 分离及理化性质]
Ann Inst Pasteur (Paris). 1961 Mar;100:377-89.
5
The fibrinogen molecule: its size, shape, and mode of polymerization.纤维蛋白原分子：其大小、形状及聚合模式。
J Biophys Biochem Cytol. 1959 Jan 25;5(1):11-6. doi: 10.1083/jcb.5.1.11.
6
Heat changes during the clotting of fibrinogen.
Nature. 1956 Nov 3;178(4540):985. doi: 10.1038/178985a0.
7
Physicochemical studies of bovine fibrinogen. II. Depolarization of fluorescence studies.牛纤维蛋白原的物理化学研究。II. 荧光去极化研究。
Biochim Biophys Acta. 1965 Jul 22;102(2):476-86. doi: 10.1016/0926-6585(65)90138-x.
8
On the primary structure of human fibrinogen. Isolation and characterization of N-terminal fragments from plasmic digests.关于人纤维蛋白原的一级结构。血浆消化产物N端片段的分离与特性鉴定。
Eur J Biochem. 1969 Mar;8(2):189-99. doi: 10.1111/j.1432-1033.1969.tb00514.x.
9
Physicochemical studies of bovine fibrinogen. IV. Ultraviolet absorption and its relation to the structure of the molecule.牛纤维蛋白原的物理化学研究。IV. 紫外线吸收及其与分子结构的关系。
Biochemistry. 1968 Jan;7(1):208-23. doi: 10.1021/bi00841a026.
10
[Electron microscopical studies on the form and macromolecular construction of fibrinogen molecules and fibrin fibers].[纤维蛋白原分子和纤维蛋白纤维的形态及大分子结构的电子显微镜研究]
Z Zellforsch Mikrosk Anat. 1967;77(4):443-517.
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