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用二硝基苯基短杆菌肽-S引发的同源抗体（兔-等电聚焦）

Homogeneous antibody elicited with dinitrophenyl-gramicidin-S (rabbit-isoelectric focusing).

作者信息

Montgomery P C, Rockey J H, Williamson A R

出版信息

Proc Natl Acad Sci U S A. 1972 Jan;69(1):228-32. doi: 10.1073/pnas.69.1.228.

DOI:10.1073/pnas.69.1.228
PMID:4536669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427581/
Abstract

Rabbit antibody to dinitrophenyl hapten has been elicited by hyperimmunization with a bifunctional cyclic decapeptide antigen, di-dinitrophenyl-gramicidin-S. Examination of the induced antibody by isoelectric focusing, and Sipsian analysis of the haptenbinding data, demonstrated that di-dinitrophenyl-gramicidin-S was capable of stimulating and maintaining the production of a biosynthetically homogeneous antibody in the adult animal.

摘要

通过用双功能环状十肽抗原二硝基苯基短杆菌肽-S进行超免疫，已诱导出兔抗二硝基苯基半抗原抗体。通过等电聚焦对诱导产生的抗体进行检测，并对半抗原结合数据进行西普西安分析，结果表明二硝基苯基短杆菌肽-S能够刺激并维持成年动物体内生物合成均一抗体的产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/427581/0a2ac1736f23/pnas00127-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/427581/6de7325f82d8/pnas00127-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/427581/0a2ac1736f23/pnas00127-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/427581/6de7325f82d8/pnas00127-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/427581/0a2ac1736f23/pnas00127-0236-a.jpg

相似文献

1
Homogeneous antibody elicited with dinitrophenyl-gramicidin-S (rabbit-isoelectric focusing).用二硝基苯基短杆菌肽-S引发的同源抗体（兔-等电聚焦）
Proc Natl Acad Sci U S A. 1972 Jan;69(1):228-32. doi: 10.1073/pnas.69.1.228.
2
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引用本文的文献

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J Exp Med. 1974 Dec 1;140(6):1468-81. doi: 10.1084/jem.140.6.1468.
2
Extent and control of antibody diversity.抗体多样性的程度与调控
Biochem J. 1972 Nov;130(2):325-33. doi: 10.1042/bj1300325.
3
The mechanism of antigenic stimulation of primary and secondary clonal precursor cells.

本文引用的文献

1
The free amino group of gramicidin S.短杆菌肽S的游离氨基
Biochem J. 1946;40(2):261-2.
2
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
3
Determination of antibody-hapten equilibrium constants by an ammonium sulfate precipitation technique.用硫酸铵沉淀技术测定抗体-半抗原平衡常数。
初级和次级克隆前体细胞的抗原刺激机制。
J Exp Med. 1972 Aug 1;136(2):241-60. doi: 10.1084/jem.136.2.241.
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Characterization of an antibody directed against a surface component of normal and pleomorphic cells of Streptococcus sanguis.一种针对血链球菌正常细胞和多形细胞表面成分的抗体的特性分析。
Infect Immun. 1975 Sep;12(3):668-78. doi: 10.1128/iai.12.3.668-678.1975.
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Alteration of clonal profile. I. Effect of sublethal irradiation on the responses to phosphorylcholine in BALB/c mice.克隆谱的改变。I. 亚致死剂量照射对BALB/c小鼠对磷酸胆碱反应的影响。
J Exp Med. 1978 Oct 1;148(4):987-95. doi: 10.1084/jem.148.4.987.
6
Hapten-specific hemolytic plaque assays usually fail to detect most of the diversity in the anti-hapten response.半抗原特异性溶血空斑试验通常无法检测出抗半抗原反应中的大多数多样性。
J Exp Med. 1977 Feb 1;145(2):372-89. doi: 10.1084/jem.145.2.372.
J Immunol. 1969 Sep;103(3):625-7.
4
Antivasopressin antibody. Characterization of high-affinity rabbit antibody with limited association constant heterogeneity.抗加压素抗体。具有有限关联常数异质性的高亲和力兔抗体的特性
Biochemistry. 1969 Jul;8(7):2719-28. doi: 10.1021/bi00835a005.
5
Antibodies of limited heterogeneity: L chains of a single mobility.有限异质性抗体：单一迁移率的轻链。
Immunochemistry. 1970 Apr;7(4):329-40. doi: 10.1016/0019-2791(70)90236-3.
6
One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins.一个细胞一种免疫球蛋白。骨髓瘤蛋白有限异质性的起源。
Biochem J. 1970 Jan;116(2):241-8. doi: 10.1042/bj1160241.
7
Induction of rabbit antibody with molecular uniformity after immunization with group C streptococci.用C组链球菌免疫后诱导产生分子均一性的兔抗体。
J Exp Med. 1970 Jan 1;131(1):207-21. doi: 10.1084/jem.131.1.207.
8
Selection of a single antibody-forming cell clone and its propagation in syngeneic mice.单个抗体形成细胞克隆的选择及其在同基因小鼠中的增殖。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1398-403. doi: 10.1073/pnas.67.3.1398.
9
Electrophoretically homogeneous anti-DNP antibodies with restricted isoelectric points elicited in mice by immunization with the antigen papain-S-DNPL.通过用抗原木瓜蛋白酶-S-DNPL免疫小鼠产生的具有受限等电点的电泳纯抗-DNP抗体。
Proc Natl Acad Sci U S A. 1970 Jun;66(2):411-8. doi: 10.1073/pnas.66.2.411.
10
Amino acid sequence restriction in rabbit antibody light chains.兔抗体轻链中的氨基酸序列限制
Proc Natl Acad Sci U S A. 1969 Jul;63(3):890-6. doi: 10.1073/pnas.63.3.890.